Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Multispecies AKT/ERK Activation InstantOne™ ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Human Arginase-1 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 8.5% |
| Intraassay CV | 6.6% |
| Gene Symbol | ARG1 |
| Total Assay Time | 3 hr 30 min |
| Sample Volume | Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL |
| Assay Sensitivity | 20.81 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 78.1-5000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P05089 |
| Regulatory Status | RUO |
| Target | Arginase 1 |
| Kit Contents | Pre-coated 96 well plate, Standard (2 vials), Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers,Standard (2 vials),Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,Streptavidin-HRP,Wash Buffer,Substrate Solution,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 383 |
| Sample Type | Cell Lysates,Plasma,Serum |
| Label or Dye | Biotin |
Invitrogen™ Mouse GFAP ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 14580 |
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Assay Sensitivity | 9.38 pg/mL |
| Assay Range | 15.63 to 1000 pg/mL |
Invitrogen™ Human IgG (Total) Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL |
| Assay Sensitivity | 1.6 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 1.6 to 100 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Human IgG |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum |
| Label or Dye | HRP |
| Synonym | Immunoglobulin G |
Invitrogen™ Mouse IL-17 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Interassay CV | 8.5% |
| Intraassay CV | 9.1% |
| Gene Symbol | IL17A |
| Total Assay Time | 3 hr 10 min |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 1.6 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 7.8-500 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | Q62386 |
| Regulatory Status | RUO |
| Target | IL-17A |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 16171 |
| Sample Type | Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | Interleukin-17, IL-17 A, CTLA8 |
Invitrogen™ Mouse IL-5 Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Immunoassay Kit Format | Uncoated ELISA Kit |
|---|---|
| Content And Storage | Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10 x Coating Buffer: Buffer for plating the Capture Antibody 5 x ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Enzyme: Pre-titrated Avidin-HRP Substrate: 1 x TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96-well Plate: Corning Costar 9018 (included with product Cat. No. ending in suffixes -22, -76, -86) |
| Target Species | Mouse |
| Conjugate | HRP |
| Gene Symbol | Il5 |
| Total Assay Time | 24 hr 30 min |
| Sample Volume | Lavage Fluid 100 μL, Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity | 8 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 8-500 pg/mL |
| Test Time | 1 hr 45 min |
| Accession Number | P04401 |
| Regulatory Status | RUO |
| Target | IL-5 |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| Gene Alias | B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRFB-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF; CANDF6; IL-17F; ML-1; ML1 |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 16191 |
| Sample Type | Lavage Fluid,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Mouse IFN gamma Uncoated ELISA Kit with Plates
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Gene Symbol | BCGF-1,BCGF1,BSF-1,BSF1,IL-4 |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity | 15 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 15 to 2,000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Accession Number | P01580 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 15978 |
| Sample Type | Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Interferon-gamma |
R&D Systems™ Human Total Adiponectin/Acrp30 Quantikine ELISA
Human Total Adiponectin/Acrp30 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human total Adiponectin (low, middle, and high molecular weight) |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 9370 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA),Plasma (Heparin),Serum |
| Sample Volume | Cell Culture Supernates (50uL),Serum (10uL),EDTA Plasma (10uL),Heparin Plasma (10uL) |
| Synonym | ACDC, Acrp30, ACRP30ADPN, adipocyte, C1Q and collagen domain containing, adiponectin, adiponectin, C1Q and collagen domain containing, AdipoQ, ADIPQTL1, apM-1, ApM1, APM1APM-1, C1q and collagen domain-containing protein, GBP28, GBP28apM1, Gelatin-binding protein |
| Assay Sensitivity | 0.891ng/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 3.90 - 250ng/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules. |
Invitrogen™ Human IL-15 Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Mouse CD40L (Soluble) ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Intraassay CV | 6.5% |
| Total Assay Time | 3 hr 10 min |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.14 ng/mL |
| Assay Range | 0.31-20.0 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Regulatory Status | RUO |
| Target | CD154 (CD40 Ligand) |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 21947 |
| Sample Type | Serum,Supernatant |
| Synonym | TRAP, HIGM 1, T cell antigen Gp39, CD154 |
R&D Systems™ Human IL-6 Quantikine HS ELISA
Human IL-6 Quantikine HS ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Invitrogen™ Human 4-1BB Ligand ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | TNFSF9 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.2 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.2 to 50 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 4 1 BB Ligand; 4 1 BBL; 41 BB Ligand; 4-1 BB ligand; 41 BBL; 4-1 BBL; 4-1 BB-L; AI848817; CD 137 Ligand; CD 137 L; CD137 Ligand; CD137L; Cd157l; CDw137L; costimulatory molecule; homolog of mouse 4-1 BB-L; Ly63l; receptor 4-1 BB ligand; TNFRSF9; Tnfsf9; TNLG5A; tumor necrosis factor (ligand) superfamily member 9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand 5 A; tumor necrosis factor ligand superfamily member 9; tumor necrosis factor superfamily member 9 |
| Regulatory Status | RUO |
| Target | 4-1 BBL (CD137L) |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 8744 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human TNF alpha Uncoated ELISA Kit with Plates
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Gene Symbol | GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 100 μL, Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity | 4 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 4 to 500 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Accession Number | P01375 |
| Regulatory Status | RUO |
| Target | TNF alpha |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 7124 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Tumor Necrosis Factor alpha, tnf-a, tnf-alpha |
Invitrogen™ Histamine Competitive ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Assay Sensitivity | 0.94 ng/mL |
| Assay Range | 1.56 to 100 ng/mL |
Invitrogen™ Human Osteoprotegerin (TNFRSF11B) Instant ELISA™ Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 4982 |
| Interassay CV | 8% |
| Intraassay CV | 7 |
| Assay Sensitivity | 2.5 pg/mL |
| Assay Range | 15.6 to 1,000 pg/mL |
| For Use With (Application) | ELISA |