Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (131,857)

Singleplex and Multiplex Bead Based Immunoassays (5,864)

ELISA Substrates and Reagents (2,338)

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241 – 255 of 137,516 results

241

Invitrogen™ Human IgM Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	15.6 ng/mL
Assay Range	15.6 to 1,000 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Human IgM
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Human IgM for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3507
Sample Type	Plasma,Serum,Supernatant
Synonym	Immunoglobulin M

242

Invitrogen™ Mouse MCP-1/CCL2 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	Ccl2
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	15 pg/mL
Storage Requirements	2-8°C
Assay Range	15-2000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P10148
Regulatory Status	RUO
Target	CCL2 (MCP-1)
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	20296
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Monocyte chemotactic protein 1

243

Invitrogen™ Mouse Ig Isotyping Uncoated ELISA Kit

ELISA

Pricing & Availability

244

Invitrogen™ Human IgG (Total) Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL
Assay Sensitivity	1.6 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	1.6 to 100 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Human IgG
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Plasma,Serum
Label or Dye	HRP
Synonym	Immunoglobulin G

245

Invitrogen™ Human IL-6 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	DER4,Fit-1,Ly84,ST2L,St2,St2-rs1,T1,T1/ST2
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 200 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P05231
Regulatory Status	RUO
Target	IL-6
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3569
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-6

246

Invitrogen™ Human TNF alpha Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Tumor Necrosis Factor alpha, tnf-a, tnf-alpha

247

Invitrogen™ Mouse TNF alpha Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	CLMF,CLMF2,IL-12 B,IMD28,IMD29,NKSF,NKSF2,CLMF,IL-12 A,NFSK,NKSF1,P35
Total Assay Time	24 hrs 30 mins
Sample Volume	Lavage Fluid 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Tissue Homogenate 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	8 to 1,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P06804
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21926
Sample Type	Lavage Fluid,Plasma,Serum,Supernatant,Tissue Homogenates
Label or Dye	HRP
Synonym	Tumor Necrosis Factor alpha, tnf-a,tnf alpha

248

Invitrogen™ Mouse IFN gamma Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	15 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15 to 2,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P01580
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	15978
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interferon-gamma

249

Invitrogen™ Human Myeloperoxidase Instant ELISA™ Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	4353
Interassay CV	9.5%
Intraassay CV	6.2
Assay Sensitivity	26 pg/mL
Assay Range	156 to 10,000 pg/mL
For Use With (Application)	ELISA

250

Invitrogen™ Human IFN alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	7.2%
Intraassay CV	4
Gene Symbol	IFL,IFN,IFN-ALPHA,IFN-alphaD3,IFNA@,IFNA1,IFN-alphaA,IFNAB,INFA2,IFNA2
Total Assay Time	2 hrs 10 mins
Sample Volume	Plasma 20 μL, Serum 20 μL, Supernatant 20 μL
Assay Sensitivity	3.2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	7.8 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P01562,P01563
Regulatory Status	RUO
Target	IFN alpha
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3439, 3440
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Alpha interferon, IFN leukocytic, LeIF D

251

Invitrogen™ Human Cu/ZnSOD ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	5.8%
Intraassay CV	5.1
Gene Symbol	ALS,ALS1,HEL-S-44,IPOA,SOD,hSod1,homodimer
Total Assay Time	1 hrs 10 mins
Sample Volume	Amniotic Fluid 10 μL, Fetal Umbilical Vein Blood 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Urine 10 μL
Assay Sensitivity	0.04 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.08 to 5.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P00441
Regulatory Status	RUO
Target	SOD1
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	6647
Sample Type	Amniotic Fluid,Fetal Umbilical Vein Blood,Plasma,Serum,Supernatant,Urine
Label or Dye	HRP
Synonym	Cu/Zn Superoxide Dismutase

252

Invitrogen™ Human Ghrelin ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	51738
Interassay CV	8.5%
Intraassay CV	6
Assay Sensitivity	11.8 pg/mL
Assay Range	15.6 to 1,000.0 pg/mL
For Use With (Application)	ELISA

253

Invitrogen™ Rat IL-1 beta ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Rat
Conjugate	HRP
Interassay CV	10%
Intraassay CV	10
Gene Symbol	Il1b
Total Assay Time	3 hrs 10 mins
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	4.0 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	31.3 to 2,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	Q63264
Regulatory Status	RUO
Target	IL-1 beta
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	24494
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Catabolin, Interleukin-1 beta, IL1F2

254

Invitrogen™ Human TNF alpha Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	CANDF6,IL-17 F,ML-1,ML1
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Tumor Necrosis Factor alpha, tnf-a, tnf-alpha

255

Invitrogen™ Multispecies AKT/ERK Activation InstantOne™ ELISA Kit

ELISA

Pricing & Availability

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